Nadeshiko League Division 2 saw two changes from 2008, reverting back to its situation in 2007. The teams would again play each other thrice, and due to TASAKI Perule's withdrawal, both divisions would again have just eight teams. The league ran from April 11th until November 1st.
The league was a straightforward round robin tournament, again using the system of three points for a win, one for a draw and none for a loss. The teams would play each other thrice.
From 2010 the league would be reorgansied. Nadeshiko League Division 1 would be simply called the Nadeshiko League, and Division 2 would become the Challenge League.
The Nadeshiko League would be made up of ten teams, so the top two teams in Division 2 would automatically be promoted. The third-placed team would play-off against the team finishing bottom in Division 1. The Challenge League would be made up of twelve teams, divided into geographical divisions, East and West, with six teams in each. Each division would be run separately, with no inter-divisional games to be played. It was therefore necessary to introduce six non-league sides into the Challenge League through a series of play-offs.
AS Elfen Sayama FC won the division and were automatically promoted along with Iga FC Kunoichi.
Fukuoka J. Anclas, who finished third in Division 2, proceeded to play for the right to play in the Nadeshiko League in 2010 against Speranza FC Takatsuki who finished bottom in Division 1. They won on aggregate over two legs and were promoted.
Kagoshima Kamoike FC Asahina became a full member of the league and were renamed Je Vrille Kagoshima.
Bunnys Kyoto Soccer Club shortened their name to Bunnys Kyoto SC.
Final Standings and Results
| | Played | Won | Drawn | Lost | GF | GA | +/- | Points |
| 1 | AS Elfen Sayama FC | 21 | 17 | 1 | 3 | 92 | 23 | 69 | 52 |
| 2 | Iga FC Kunoichi | 21 | 17 | 0 | 4 | 84 | 13 | 71 | 51 |
| 3 | Fukuoka J. Anclas | 21 | 16 | 1 | 4 | 88 | 18 | 70 | 49 |
| 4 | Shimizu Daihachi Pleiades | 21 | 6 | 7 | 8 | 29 | 22 | 7 | 25 |
| 5 | Ohara Gakuen JaSRA SC | 21 | 7 | 4 | 10 | 26 | 39 | -13 | 25 |
| 6 | Bunnys Kyoto SC | 21 | 4 | 5 | 12 | 16 | 46 | -30 | 17 |
| 7 | Je Vrille Kagoshima | 21 | 5 | 2 | 14 | 22 | 58 | -36 | 17 |
| 8 | Renaissance Kumamoto FC | 21 | 1 | 2 | 18 | 12 | 150 | -138 | 5 |
First Round, games played April 11th and 12th
Ohara Gakuen JaSRA SC 0 - 0 Shimizu Daihachi Pleiades
(Attendance: 200)
Iga FC Kunoichi 12 - 0 Renaissance Kumamoto F.C.
(Attendance: 270)
AS Elfen Sayama FC 4 - 3 Je Vrille Kagoshima
(Attendance: 252)
Fukuoka J. Anclas 2 - 0 Bunnys Kyoto SC
(Attendance: 485)
Second Round, games played April 19th
Bunnys Kyoto SC 1 - 0 Ohara Gakuen JaSRA SC
(Attendance: 110)
Fukuoka J. Anclas 10 - 0 Renaissance Kumamoto F.C.
(Attendance: 508)
Je Vrille Kagoshima 0 - 11 Iga FC Kunoichi
(Attendance: 792)
Shimizu Daihachi Pleiades 0 - 1 AS Elfen Sayama FC
(Attendance: 263)
Third Round, games played April 26th
AS Elfen Sayama FC 2 - 0 Bunnys Kyoto SC
(Attendance: 230)
Ohara Gakuen JaSRA SC 0 - 5 Fukuoka J. Anclas
(Attendance: 100)
Iga FC Kunoichi 1 - 0 Shimizu Daihachi Pleiades
(Attendance: 230)
Renaissance Kumamoto F.C. 0 - 3 Je Vrille Kagoshima
(Attendance: 200)
Fourth Round, games played May 2nd
Renaissance Kumamoto F.C. 1 - 5 Shimizu Daihachi Pleiades
(Attendance: 150)
AS Elfen Sayama FC 1 - 0 Ohara Gakuen JaSRA SC
(Attendance: 123)
Iga FC Kunoichi 3 - 1 Fukuoka J. Anclas
(Attendance: 210)
Je Vrille Kagoshima 0 - 1 Bunnys Kyoto SC
(Attendance: 580)
Fifth Round, games played May 6th
Ohara Gakuen JaSRA SC 2 - 0 Renaissance Kumamoto F.C.
(Attendance: 100)
Fukuoka J. Anclas 2 - 0 AS Elfen Sayama FC
(Attendance: 614)
Shimizu Daihachi Pleiades 1 - 1 Je Vrille Kagoshima
(Attendance: 432)
Bunnys Kyoto SC 0 - 2 Iga FC Kunoichi
(Attendance: 225)
Sixth Round, games played May 10th
Shimizu Daihachi Pleiades 2 - 0 Bunnys Kyoto SC
(Attendance: 430)
Renaissance Kumamoto F.C. 2 - 8 AS Elfen Sayama FC
(Attendance: 120)
Iga FC Kunoichi 3 - 0 Ohara Gakuen JaSRA SC
(Attendance: 330)
Je Vrille Kagoshima 1 - 3 Fukuoka J. Anclas
(Attendance: 460)
Seventh Round, games played May 17th
Ohara Gakuen JaSRA SC 1 - 0 Je Vrille Kagoshima
(Attendance: 50)
Bunnys Kyoto SC 1 - 1 Renaissance Kumamoto F.C.
(Attendance: 210)
Fukuoka J. Anclas 1 - 1 Shimizu Daihachi Pleiades
(Attendance: 453)
AS Elfen Sayama FC 1 - 3 Iga FC Kunoichi
(Attendance: 402)
Eighth Round, games played June 7th
Renaissance Kumamoto F.C. 0 - 9 Iga FC Kunoichi
(Attendance: 522)
Je Vrille Kagoshima 1 - 5 AS Elfen Sayama FC
(Attendance: 300)
Shimizu Daihachi Pleiades 1 - 1 Ohara Gakuen JaSRA SC
(Attendance: 527)
Bunnys Kyoto SC 0 - 4 Fukuoka J. Anclas
(Attendance: 100)
Ninth Round, games played June 13th and 14th
Ohara Gakuen JaSRA SC 1 - 2 Bunnys Kyoto SC
(Attendance: 150)
AS Elfen Sayama FC 3 - 1 Shimizu Daihachi Pleiades
(Attendance: 184)
Iga FC Kunoichi 1 - 0 Je Vrille Kagoshima
(Attendance: 430)
Renaissance Kumamoto F.C. 0 - 17 Fukuoka J. Anclas
(Attendance: 330)
Tenth Round, games played June 21st
Fukuoka J. Anclas 4 - 3 Ohara Gakuen JaSRA SC
(Attendance: 315)
Shimizu Daihachi Pleiades 1 - 0 Iga FC Kunoichi
(Attendance: 132)
Bunnys Kyoto SC 0 - 4 AS Elfen Sayama FC
(Attendance: 150)
Je Vrille Kagoshima 6 - 1 Renaissance Kumamoto F.C.
(Attendance: 1,600)
Eleventh Round, games played June 28th
Renaissance Kumamoto F.C. 2 - 3 Ohara Gakuen JaSRA SC
(Attendance: 150)
Je Vrille Kagoshima 0 - 0 Shimizu Daihachi Pleiades
(Attendance: 380)
AS Elfen Sayama FC 3 - 2 Fukuoka J. Anclas
(Attendance: 281)
Iga FC Kunoichi 5 - 0 Bunnys Kyoto SC
(Attendance: 370)
Twelfth Round, games played July 4th and 5th
Bunnys Kyoto SC 3 - 1 Je Vrille Kagoshima
(Attendance: 50)
Shimizu Daihachi Pleiades 8 - 0 Renaissance Kumamoto F.C.
(Attendance: 547)
Ohara Gakuen JaSRA SC 3 - 3 AS Elfen Sayama FC
(Attendance: 100)
Fukuoka J. Anclas 2 - 0 Iga FC Kunoichi
(Attendance: 415)
Thirteenth Round, games played July 12th
AS Elfen Sayama FC 20 - 1 Renaissance Kumamoto F.C.
(Attendance: 281)
Ohara Gakuen JaSRA SC 1 - 3 Iga FC Kunoichi
(Attendance: 200)
Bunnys Kyoto SC 1 - 1 Shimizu Daihachi Pleiades
(Attendance: 130)
Fukuoka J. Anclas 7 - 0 Je Vrille Kagoshima
(Attendance: 492)
Fourteenth Round, games played July 19th
Iga FC Kunoichi 3 - 2 AS Elfen Sayama FC
(Attendance: 360)
Je Vrille Kagoshima 1 - 3 Ohara Gakuen JaSRA SC
(Attendance: 497)
Renaissance Kumamoto F.C. 2 - 1 Bunnys Kyoto SC
(played July 26th)
(Attendance: 180)
Shimizu Daihachi Pleiades 1 - 3 Fukuoka J. Anclas
(played August 30th)
(Attendance: 223)
Fifteenth Round, games played September 5th and 6th
Ohara Gakuen JaSRA SC 1 - 0 Shimizu Daihachi Pleiades
(Attendance: 100)
AS Elfen Sayama FC 7 - 0 Je Vrille Kagoshima
(Attendance: 285)
Iga FC Kunoichi 12 - 0 Renaissance Kumamoto F.C.
(Attendance: 220)
Fukuoka J. Anclas 4 - 1 Bunnys Kyoto SC
(Attendance: 194)
Sixteenth Round, games played September 13th
Bunnys Kyoto SC 2 - 2 Ohara Gakuen JaSRA SC
(Attendance: 90)
Je Vrille Kagoshima 0 - 7 Iga FC Kunoichi
(Attendance: 1,423)
Shimizu Daihachi Pleiades 0 - 2 AS Elfen Sayama FC
(Attendance: 256)
Renaissance Kumamoto F.C. 1 - 13 Fukuoka J. Anclas
(Attendance: 317)
Seventeenth Round, games played September 20th and 21st
AS Elfen Sayama FC 8 - 1 Bunnys Kyoto SC
(Attendance: 262)
Ohara Gakuen JaSRA SC 0 - 4 Fukuoka J. Anclas
(Attendance: 50)
Renaissance Kumamoto F.C. 0 - 3 Je Vrille Kagoshima
(Attendance: 220)
Iga FC Kunoichi 3 - 0 Shimizu Daihachi Pleiades
(Attendance: 600)
Eighteenth Round, games played October 11th
Shimizu Daihachi Pleiades 1 - 0 Je Vrille Kagoshima
(Attendance: 156)
AS Elfen Sayama FC 2 - 1 Fukuoka J. Anclas
(Attendance: 281)
Renaissance Kumamoto F.C. 0 - 2 Ohara Gakuen JaSRA SC
(Attendance: 260)
Bunnys Kyoto SC 1 - 3 Iga FC Kunoichi
(Attendance: 250)
Nineteenth Round, games played October 17th and 18th
Ohara Gakuen JaSRA SC 0 - 5 AS Elfen Sayama FC
(Attendance: 50)
Renaissance Kumamoto F.C. 1 - 5 Shimizu Daihachi Pleiades
(Attendance: 100)
Je Vrille Kagoshima 1 - 0 Bunnys Kyoto SC
(Attendance: 370)
Fukuoka J. Anclas 2 - 1 Iga FC Kunoichi
(Attendance: 726)
Twentieth Round, games played October 25th
Shimizu Daihachi Pleiades 1 - 1 Bunnys Kyoto SC
(Attendance: 372)
AS Elfen Sayama FC 10 - 0 Renaissance Kumamoto F.C.
(Attendance: 197)
Iga FC Kunoichi 2 - 1 Ohara Gakuen JaSRA SC
(Attendance: 310)
Je Vrille Kagoshima 1 - 0 Fukuoka J. Anclas
(Attendance: 320)
Twenty-First Round, games played November 1st
Ohara Gakuen JaSRA SC 2 - 0 Je Vrille Kagoshima
(Attendance: 250)
Iga FC Kunoichi 0 - 1 AS Elfen Sayama FC
(Attendance: 380)
Bunnys Kyoto SC 0 - 0 Renaissance Kumamoto F.C.
(Attendance: 140)
Fukuoka J. Anclas 1 - 0 Shimizu Daihachi Pleiades
(Attendance: 1,124)
Promotion/Relegation Play-off, games played November 8th and 15th
Speranza FC Takatsuki 0 - 3 Fukuoka J. Anclas
(Attendance: 628)
Fukuoka J. Anclas 0 - 1 Speranza FC Takatsuki
(Attendance: 1,548)
Fukuoka J. Anclas win 3-1 on aggregate.
